GRP ELISA Kit (Mouse)

• Reproducibility :

  Inter-Assay CV

• CAS Number :

  7732-18-5

• Gene Name :

  Gastrin releasing peptide

• Gene Aliases :

  B; BLP; gastrin-releasing peptide.

• Gene ID :

  225642

• Accession Number :

  NP_778177

• Reactivity :

  Mouse, Mus musculus

• Target :

  Gastrin-releasing peptide

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle :

  Aviva Systems Biology Grp ELISA Kit (Mouse) (OKEH06991) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Grp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Grp and incubated. The Grp found in the sample or standards competes with the biotinylated Grp for limited binding sites on the immobilized anti-Grp antibody. Excess unbound biotinylated Grp and sample or standard Grp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Grp captured in the well and inversely proportional to the amount of Grp which was contained in the sample or standard.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, Plasma, Tissue Homogenates

• Detection Range :

  0.312-20ng/mL

• Recovery :

  Mean recovery when spiking into matrix = 88%

• Sensitivity :

  0.156 ng/mL

• Reconstitution :

  Store as indicated in product manual.

• Specificity :

  Natural and recombinant Mouse Gastrin-releasing peptide

• NCBI Gene Symbol :

  Grp

• Protein Name :

  Q8R1I2

• Gene Name URL :

  Grp

• Nucleotide Accession Number :

  NM_175012