GRP ELISA Kit (Rat)

CAT: 0247-OKEH06339-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH06339-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Gastrin releasing peptide
Gene Aliases
Gastrin-releasing peptide.
Gene ID
171101
Accession Number
NP_001020241
Reactivity
Rat, Rattus norvegicus
Target
Gastrin-releasing peptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Grp ELISA Kit (Rat) (OKEH06339) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Grp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Grp and incubated. The Grp found in the sample or standards competes with the biotinylated Grp for limited binding sites on the immobilized anti-Grp antibody. Excess unbound biotinylated Grp and sample or standard Grp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Grp captured in the well and inversely proportional to the amount of Grp which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 95%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Gastrin-releasing peptide
NCBI Gene Symbol
Grp
Protein Name
P24393
Gene Name URL
Grp
Nucleotide Accession Number
NM_001025070

Related Products

CatalogName
OKEH06991-96WGRP ELISA Kit (Mouse)
OKCA02384-96WGRP ELISA Kit (Mouse)
OKEH00885-96WGRP ELISA Kit (Human)
OKCA02384GRP ELISA Kit (Mouse) (OKCA02384)
OKEH06991GRP ELISA Kit (Mouse) (OKEH06991)
OKEH00885GRP ELISA Kit (Human) : 96 Wells (OKEH00885)
OACA03069-50UGGRP Antibody
OAAN01767-50ULGRP Antibody
OACA03070-100UGGRP Antibody
OACA03070-50UGGRP Antibody

Alternative Products

CatalogName
GTR10468219Rat Cholesterol ELISA ELISA
1036Rat Nephrin ELISA
DIY0750R-01Rat VEGF-A ELISA
ERN0048Rat NO ELISA Kit
ERA0046Rat Aβ ELISA Kit
ERI0046Rat IS ELISA Kit
MBS494518-01CRP (Rat) ELISA
MBS495068-01Estradiol (Rat) ELISA
KT-30309Rat TGFb1 ELISA
KT-51483Rat Podocalyxin ELISA

Popular Products