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GOT1 ELISA Kit (Human)

CAT: 0247-OKEH06988-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06988-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Glutamic-oxaloacetic transaminase 1
Gene Aliases
Aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; GIG18; glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; transaminase A.
Gene ID
2805
Accession Number
NP_002070
Reactivity
Homo sapiens, Human
Target
Aspartate aminotransferase, cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GOT1 ELISA Kit (Human) (OKEH06988) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GOT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GOT1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56-100U/L
Recovery
Mean recovery when spiking into matrix = 95%
Sensitivity
0.87U/L
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Aspartate aminotransferase, cytoplasmic
NCBI Gene Symbol
GOT1
Protein Name
P17174
Gene Name URL
GOT1
Nucleotide Accession Number
NM_002079

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