GOT1 ELISA Kit (Human)

• Reproducibility:

  Inter-Assay CV

• Gene Name:

  Glutamic-oxaloacetic transaminase 1

• Gene Aliases:

  Aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; GIG18; glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; transaminase A.

• Gene ID:

  2805

• Accession Number:

  NP_002070

• Reactivity:

  Homo sapiens, Human

• Target:

  Aspartate aminotransferase, cytoplasmic

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology GOT1 ELISA Kit (Human) (OKEH06988) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GOT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GOT1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  1.56-100U/L

• Recovery:

  Mean recovery when spiking into matrix = 95%

• Sensitivity:

  0.87U/L

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Aspartate aminotransferase, cytoplasmic

• NCBI Gene Symbol:

  GOT1

• Protein Name:

  P17174

• Gene Name URL:

  GOT1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_002079