GOT1 ELISA Kit (Rat)

CAT: 0247-OKEH03319-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03319-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Glutamic-oxaloacetic transaminase 1
Gene Aliases
AAT1; ASAT; aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; Aspat; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Gaspat; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; transaminase A.
Gene ID
24401
Accession Number
NP_036703.2
Reactivity
Rat, Rattus norvegicus
Target
Aspartate aminotransferase, cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GOT1 ELISA Kit (Rat) (OKEH03319) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Got1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Got1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Got1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200U/L
Sensitivity
1.63U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Rat Aspartate aminotransferase, cytoplasmic
Protein Length
413
NCBI Gene Symbol
Got1
Protein Name
P13221
Gene Name URL
Got1
Nucleotide Accession Number
NM_012571.2

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