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EPAS1 ELISA Kit (Mouse)

CAT: 0247-OKEH06956-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06956-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Endothelial PAS domain protein 1
Gene Aliases
BHLHe7; bHLHe73; endothelial PAS domain-containing protein 1; EPAS-1; HIF; Hif like protein; HIF1 alpha-like factor; HIF1alpha-like factor; HIF-1-alpha-like factor; HIF-2; HIF2A; HIF2-alpha; HIF-2alpha; HIF-2-alpha; HIF-related factor; HL; HLF; HLF (HIF1alpha-like factor) ; HR; HRF; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2-alpha; mHLF; MO; MOP2.
Gene ID
13819
Accession Number
NP_034267
Reactivity
Mouse, Mus musculus
Target
Endothelial PAS domain-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Epas1 ELISA Kit (Mouse) (OKEH06956) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Epas1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Epas1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Epas1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 89.8%
Sensitivity
39.7 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Endothelial PAS domain-containing protein 1
NCBI Gene Symbol
Epas1
Protein Name
P97481
Gene Name URL
Epas1
Nucleotide Accession Number
NM_010137

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