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CEBPB ELISA Kit (Human)

CAT: 0247-OKEH06900-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06900-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
CCAAT enhancer binding protein beta
Gene Aliases
C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; IL6DBP; interleukin 6-dependent DNA-binding protein; Liver activator protein; Liver-enriched inhibitory protein; NF-IL6; nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5; transcription factor 5; transcription factor C/EBP beta.
Gene ID
1051
Accession Number
NP_001272807
Reactivity
Homo sapiens, Human
Target
CCAAT/enhancer-binding protein beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CEBPB ELISA Kit (Human) (OKEH06900) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEBPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEBPB captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 96%
Sensitivity
39.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human CCAAT/enhancer-binding protein beta
NCBI Gene Symbol
CEBPB
Protein Name
P17676
Gene Name URL
CEBPB
Nucleotide Accession Number
NM_001285878

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