CAPRIN1 ELISA Kit (Human)

CAT: 0247-OKEH06879-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH06879-96WSize:96 Well
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Cell cycle associated protein 1
Gene Aliases
Activation/proliferation-associated protein 1; caprin 1; caprin-1; Cell cycle-associated protein 1; cytoplasmic activation- and proliferation-associated protein 1; cytoplasmic activation/proliferation-associated protein-1; GPI-anchored membrane protein 1; GPI-anchored protein p137; GPIAP1; GPI-p137; GPIP137; GRIP137; M11S1; membrane component chromosome 11 surface marker 1; p137GPI; RNA granule protein 105; RNG105.
Gene ID
4076
Accession Number
NP_005889
Reactivity
Homo sapiens, Human
Target
Caprin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAPRIN1 ELISA Kit (Human) (OKEH06879) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPRIN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPRIN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPRIN1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 106%
Sensitivity
0.44 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Caprin-1
NCBI Gene Symbol
CAPRIN1
Protein Name
Q14444
Gene Name URL
CAPRIN1
Nucleotide Accession Number
NM_005898

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