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ADAM10 ELISA Kit (Mouse)

CAT: 0247-OKEH06827-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06827-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Reproducibility CV% < 7.1% (n=20) < br>Inter-Assay Reproducibility CV% < 9.2% (n=24)
CAS Number
7732-18-5
Gene Name
A disintegrin and metallopeptidase domain 10
Gene Aliases
1700031C13Rik; a disintegrin and metalloprotease domain (ADAM) 10; disintegrin and metalloproteinase domain-containing protein 10; kuz; kuzb; kuzbanian; kuzbanian protein homolog; MADM; mammalian disintegrin-metalloprotease.
Gene ID
11487
Accession Number
NP_031425
Reactivity
Mouse, Mus musculus
Target
Disintegrin and metalloproteinase domain-containing protein 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Adam10 ELISA Kit (Mouse) (OKEH06827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adam10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adam10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adam10 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10
NCBI Gene Symbol
Adam10
Protein Name
O35598
Gene Name URL
Adam10
Nucleotide Accession Number
NM_007399

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