CDK12 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Reproducibility CV% < 4.6% (n=20) < br>Inter-Assay Reproducibility CV% < 7.6% (n=24)

• Gene Name:

  Cyclin-dependent kinase 12

• Gene Aliases:

  1810022J16Rik; AI646528; CDC2-related kinase 7; CDC2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; cell division cycle 2-related protein kinase 7; cell division protein kinase 12; Cr; Crk; Crk7; Crkrs; cyclin-dependent kinase 12; D11Ertd752; D11Ertd752e; Pksc; protein kinase for splicing component.

• Gene ID:

  69131

• Accession Number:

  NP_001103096

• Reactivity:

  Mouse, Mus musculus

• Target:

  Cyclin-dependent kinase 12

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Cdk12 ELISA Kit (Mouse) (OKEH06794) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk12 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  78-5000pg/ml

• Recovery:

  Mean recovery when spiking into serum = 96.6%

• Sensitivity:

  78 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  Cdk12

• Protein Name:

  Q14AX6

• Gene Name URL:

  Cdk12

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001109626