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CDK12 ELISA Kit (Mouse)

CAT: 0247-OKEH06794-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH06794-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Reproducibility CV% < 4.6% (n=20) < br>Inter-Assay Reproducibility CV% < 7.6% (n=24)
CAS Number
7732-18-5
Gene Name
Cyclin-dependent kinase 12
Gene Aliases
1810022J16Rik; AI646528; CDC2-related kinase 7; CDC2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; cell division cycle 2-related protein kinase 7; cell division protein kinase 12; Cr; Crk; Crk7; Crkrs; cyclin-dependent kinase 12; D11Ertd752; D11Ertd752e; Pksc; protein kinase for splicing component.
Gene ID
69131
Accession Number
NP_001103096
Reactivity
Mouse, Mus musculus
Target
Cyclin-dependent kinase 12
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cdk12 ELISA Kit (Mouse) (OKEH06794) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk12 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into serum = 96.6%
Sensitivity
78 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Cdk12
Protein Name
Q14AX6
Gene Name URL
Cdk12
Nucleotide Accession Number
NM_001109626

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