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CDK12 ELISA Kit (Rat)

CAT: 0247-OKEH05976-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05976-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Cyclin-dependent kinase 12
Gene Aliases
Crk7, Pksc, Crkrs
Gene ID
192350
Accession Number
NP_001029039.1
Reactivity
Rat
Target
Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Cdk12 ELISA Kit (Rat) (OKEH05976) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk12 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cdk12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk12 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Cyclin-dependent kinase 12
Protein Length
1484
NCBI Gene Symbol
CDK12
Protein Name
Cyclin-dependent kinase 12
Gene Name URL
CDK12
Nucleotide Accession Number
NM_001033867.1

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