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HILPDA ELISA Kit (Mouse)

CAT: 0247-OKEH06764-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06764-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Reproducibility CV% < 8.8% (n=20) < br>Inter-Assay Reproducibility CV% < 10.6% (n=24)
CAS Number
7732-18-5
Gene Name
Hypoxia inducible lipid droplet associated
Gene Aliases
2310016C08Rik; AW049004; Hig2; hypoxia-inducible gene 2 protein; hypoxia-inducible lipid droplet-associated protein.
Gene ID
69573
Accession Number
NP_076005
Reactivity
Mouse, Mus musculus
Target
Hypoxia-inducible lipid droplet-associated protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hilpda ELISA Kit (Mouse) (OKEH06764) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hilpda has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hilpda is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hilpda captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into serum = 102.6%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Hilpda
Protein Name
Q9JLS0
Gene Name URL
Hilpda
Nucleotide Accession Number
NM_023516

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