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PTCH2 ELISA Kit (Human)

CAT: 0247-OKEH06557-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06557-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Patched 2
Gene Aliases
Patched homolog 2; protein patched homolog 2; PTC2.
Gene ID
8643
Accession Number
NP_001092271
Reactivity
Homo sapiens, Human
Target
Protein patched homolog 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology PTCH2 ELISA Kit (Human) (OKEH06557) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTCH2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTCH2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTCH2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 96%
Sensitivity
0.178 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Protein patched homolog 2
NCBI Gene Symbol
PTCH2
Protein Name
Q9Y6C5
Gene Name URL
PTCH2
Nucleotide Accession Number
NM_001098801

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