Products for Research Use Only
RegisterLogin

PTCH2 ELISA Kit (Mouse)

CAT: 0247-OKEH06522-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH06522-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Patched 2
Gene Aliases
Patched homolog 2; protein patched homolog 2; ptc; ptc2.
Gene ID
19207
Accession Number
NP_001160091
Reactivity
Mouse, Mus musculus
Target
Protein patched homolog 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ptch2 ELISA Kit (Mouse) (OKEH06522) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Ptch2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ptch2 and incubated. The Ptch2 found in the sample or standards competes with the biotinylated Ptch2 for limited binding sites on the immobilized anti-Ptch2 antibody. Excess unbound biotinylated Ptch2 and sample or standard Ptch2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ptch2 captured in the well and inversely proportional to the amount of Ptch2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86.1%
Sensitivity
0.169 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Protein patched homolog 2
NCBI Gene Symbol
Ptch2
Protein Name
O35595
Gene Name URL
Ptch2
Nucleotide Accession Number
NM_001166619

Related Products

CatalogName
OKEH06557-96WPTCH2 ELISA Kit (Human)
OKEH06557PTCH2 ELISA Kit (Human) (OKEH06557)
OCOA13456-20UGPTCH2 Protein Lysate
OABB00810-100UGPTCH2 Polyclonal Antibody
AAP45522-100UGPTCH2 Peptide (AAP45522)
ARP45522_P050-25ULPTCH2 Antibody - N-terminal region
ARP45522_P050PTCH2 antibody - N-terminal region (ARP45522_P050)
ARP45522_P050-FITCPTCH2 Antibody - N-terminal region : FITC (ARP45522_P050-FITC)
ARP45522_P050-BiotinPTCH2 Antibody - N-terminal region : Biotin (ARP45522_P050-Biotin)
ARP45522_P050-HRPPTCH2 Antibody - N-terminal region : HRP (ARP45522_P050-HRP)

Alternative Products

CatalogName
EF015805Mouse Ptch2 ELISA Kit
ELI-35900mMouse Protein patched homolog 2, Ptch2 ELISA KIT
AE25302MO-01Mouse Protein patched homolog 2 (PTCH2) ELISA Kit
G3819Protein patched homolog 2 (PTCH2) Mouse ELISA Kit
GTR19298964-01PTCH2 siRNA (Mouse)
38063054PTCH2 Adenovirus (Mouse)
abx930253-01Mouse PTCH2 siRNA
EF002154Human PTCH2 ELISA KIT
abx972259-01Mouse PTCH2 shRNA Plasmid
GTR15246498Lentiviral mouse Ptch2 shRNA (UAS) - Lentiviral mouse Ptch2 shRNA (UAS) (100)

Popular Products