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LCTL ELISA Kit (Human)

CAT: 0247-OKEH06555-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06555-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Lactase like
Gene Aliases
Gammaklotho; KL lactase phlorizin hydrolase; KLG; klotho gamma; klotho/lactase-phlorizin hydrolase-related protein; KLPH; lactase-like protein.
Gene ID
197021
Accession Number
NP_036199
Reactivity
Homo sapiens, Human
Target
Lactase-like protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LCTL ELISA Kit (Human) (OKEH06555) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCTL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCTL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCTL captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 89%
Sensitivity
0.339 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Lactase-like protein
NCBI Gene Symbol
LCTL
Protein Name
Q6UWM7
Gene Name URL
LCTL
Nucleotide Accession Number
NM_012067

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