LCTL ELISA Kit (Mouse)

CAT: 0247-OKEH06523-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH06523-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Lactase-like
Gene Aliases
E130104I05Rik; KL; klotho/lactase-phlorizin hydrolase-related protein; Klotho-LPH related; KLPH; lactase-like protein.
Gene ID
235435
Accession Number
NP_000301
Reactivity
Mouse, Mus musculus
Target
Lactase-like protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lctl ELISA Kit (Mouse) (OKEH06523) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Lctl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lctl and incubated. The Lctl found in the sample or standards competes with the biotinylated Lctl for limited binding sites on the immobilized anti-Lctl antibody. Excess unbound biotinylated Lctl and sample or standard Lctl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lctl captured in the well and inversely proportional to the amount of Lctl which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.2%
Sensitivity
0.314 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Lactase-like protein
NCBI Gene Symbol
Lctl
Protein Name
Q8K1F9
Gene Name URL
Lctl
Nucleotide Accession Number
NM_000310

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