GCDH ELISA Kit (Mouse)

Mean Inter-Assay Precision -

7732-18-5

Glutaryl-Coenzyme A dehydrogenase

9030411L18; AI266902; D17825; GCD; glutaryl-CoA dehydrogenase, mitochondrial.

270076

NP_058599

Mouse, Mus musculus

Glutaryl-CoA dehydrogenase, mitochondrial

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Gcdh ELISA Kit (Mouse) (OKEH06494) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Gcdh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gcdh and incubated. The Gcdh found in the sample or standards competes with the biotinylated Gcdh for limited binding sites on the immobilized anti-Gcdh antibody. Excess unbound biotinylated Gcdh and sample or standard Gcdh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gcdh captured in the well and inversely proportional to the amount of Gcdh which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids

0.625-40ng/mL

Mean recovery when spiking into Serum and Plasma = 88.8%

0.321 ng/mL

Store as indicated in product manual.

Natural and recombinant Mouse Glutaryl-CoA dehydrogenase, mitochondrial

Gcdh

Q60759

Gcdh