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PDIA3 ELISA Kit (Mouse)

CAT: 0247-OKEH06342-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06342-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Protein disulfide isomerase associated 3
Gene Aliases
58 kDa glucose-regulated protein;58 kDa microsomal protein;58kDa; disulfide isomerase ER-60; E; endoplasmic reticulum protein; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; Erp; ERp57; ERp60; ERp61; glucose regulated protein, 58 kDa; Grp; Grp58; P; p58; PDI; PDI-; PDI-Q2; phospholipase C, alpha; PI-PLC; PLC[a]; Plca; protein disulfide-isomerase A3.
Gene ID
14827
Accession Number
NP_033882
Reactivity
Mouse, Mus musculus
Target
Protein disulfide-isomerase A3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pdia3 ELISA Kit (Mouse) (OKEH06342) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pdia3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pdia3 and incubated. The Pdia3 found in the sample or standards competes with the biotinylated Pdia3 for limited binding sites on the immobilized anti-Pdia3 antibody. Excess unbound biotinylated Pdia3 and sample or standard Pdia3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pdia3 captured in the well and inversely proportional to the amount of Pdia3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 87.4%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Protein disulfide-isomerase A3
NCBI Gene Symbol
Pdia3
Protein Name
P27773
Gene Name URL
Pdia3
Nucleotide Accession Number
NM_009752

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