FFAR4 ELISA Kit (Rat)

• Reproducibility :

  Mean Inter-Assay Precision -

• Gene Name :

  Free fatty acid receptor 4

• Gene Aliases :

  Gpr120, O3far1

• Gene ID :

  294075

• Accession Number :

  NP_001040553.1

• Reactivity :

  Rat

• Target :

  Receptor for medium and long-chain free fatty acids (FFAs) . Signals via a G (q) /G (11) -coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects, particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2) /TAB1-dependent effect, but independent of the G (q) /G (11) -coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Mediates FFA-induced inhibition of apoptosis in enteroendocrine cells. May play a role in the regulation of adipocyte development and differentiation.

• Type :

  ELISA Kit

• Assay Principle :

  Aviva Systems Biology Ffar4 ELISA Kit (Rat) (OKEH06193) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ffar4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ffar4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ffar4 captured in the well.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  0.625 - 40ng/ml

• Recovery :

  Mean recovery when spiking into Serum and Plasma = 99%

• Sensitivity :

  0.322 ng/mL

• Reconstitution :

  Store as indicated in product manual.

• Specificity :

  Natural and recombinant Rat Omega-3 fatty acid receptor 1

• Protein Length :

  361

• NCBI Gene Symbol :

  FFAR4

• Protein Name :

  Free fatty acid receptor 4

• Gene Name URL :

  FFAR4

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001047088.1