TAB2 ELISA Kit (Rat)
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TAB2 ELISA Kit (Rat)
Reproducibility:
Mean Inter-Assay Precision -Gene Name:
TGF-beta activated kinase 1/MAP3K7 binding protein 2Gene Aliases:
Map3k7ip2; mitogen-activated protein kinase kinase kinase 7-interacting protein 2; TGF-beta-activated kinase 1 and MAP3K7-binding protein 2; TGF-beta-activated kinase 1-binding protein 2.Gene ID:
308267Accession Number:
NP_001012062.1Reactivity:
Rat, Rattus norvegicusTarget:
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2Type:
ELISA KitApplications:
Enzyme-linked Immunosorbent assay-SandwichAssay Principle:
Aviva Systems Biology Tab2 ELISA Kit (Rat) (OKEH06007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tab2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tab2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tab2 captured in well.Assay Protocol:
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type:
Serum, plasma and other biological fluidsDetection Range:
0.156-10ng/mLRecovery:
Mean recovery when spiking into Serum and Plasma = 101%Sensitivity:
0.088 ng/mLReconstitution:
Store as indicated in product manual.Specificity:
Natural and recombinant Rat TGF-beta-activated kinase 1 and MAP3K7-binding protein 2Protein Length:
693NCBI Gene Symbol:
Tab2Protein Name:
Q5U303Gene Name URL:
Tab2CAS Number:
7732-18-5Nucleotide Accession Number:
NM_001012062.1
