TAB2 ELISA Kit (Mouse)

CAT: 0247-OKEH05145-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05145-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
TGF-beta activated kinase 1/MAP3K7 binding protein 2
Gene Aliases
1110030N06Rik; A530078N03Rik; Map3k; Map3k7ip2; mitogen-activated protein kinase kinase kinase 7-interacting protein 2; mKIAA0733; Tak1 binding protein 2; TAK1-binding protein 2; TGF-beta-activated kinase 1 and MAP3K7-binding protein 2; TGF-beta-activated kinase 1-binding protein 2.
Gene ID
68652
Accession Number
NP_619608.1
Reactivity
Mouse, Mus musculus
Target
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tab2 ELISA Kit (Mouse) (OKEH05145) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tab2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tab2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tab2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 83%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
Protein Length
693
NCBI Gene Symbol
Tab2
Protein Name
Q99K90
Gene Name URL
Tab2
Nucleotide Accession Number
NM_138667.3

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