Products for Research Use Only

IK ELISA Kit (Rat)

CAT: 0247-OKEH05963-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05963-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
IK cytokine, down-regulator of HLA II
Gene Aliases
RED, CSA2
Gene ID
291659
Accession Number
NP_001005537.1
Reactivity
Rat
Target
May bind to chromatin.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Ik ELISA Kit (Rat) (OKEH05963) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ik has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ik is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ik captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78 - 50ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 91%
Sensitivity
0.407 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Protein Red
Protein Length
557
NCBI Gene Symbol
IK
Protein Name
Protein Red
Gene Name URL
IK
Nucleotide Accession Number
NM_001005537.1