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SCG5 ELISA Kit (Rat)

CAT: 0247-OKEH05932-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05932-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Secretogranin V
Gene Aliases
7B2; neuroendocrine protein 7B2; Secretogranin V; secretogranin V (7B2 protein) ; secretogranin-5; secretory granule endocrine protein I; secretory granule neuroendocrine protein 1; Secretory granule neuroendocrine, protein 1 (7B2 protein) ; Sgne1.
Gene ID
25719
Accession Number
NP_037307.1
Reactivity
Rat, Rattus norvegicus
Target
Neuroendocrine protein 7B2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Scg5 ELISA Kit (Rat) (OKEH05932) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Scg5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Scg5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Scg5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97%
Sensitivity
43 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Neuroendocrine protein 7B2
Protein Length
210
NCBI Gene Symbol
Scg5
Protein Name
P27682
Gene Name URL
Scg5
Nucleotide Accession Number
NM_013175.3

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