DUSP1 ELISA Kit (Rat)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  Dual specificity phosphatase 1

• Gene Aliases:

  3CH134;3CH134/CL100 PTPase; CL100; dual specificity protein phosphatase 1; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; mitogen-activated protein kinase phosphatase 1; Mkp1; MKP-1; oxidative stress-inducible protein tyrosine phosphatase; protein-tyrosine phosphatase CL100; protein-tyrosine phosphatase non-receptor type 16; Ptpn16.

• Gene ID:

  114856

• Accession Number:

  NP_446221.2

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  Dual specificity protein phosphatase 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Dusp1 ELISA Kit (Rat) (OKEH05930) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dusp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dusp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dusp1 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 102%

• Sensitivity:

  0.156 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Rat Dual specificity protein phosphatase 1

• Protein Length:

  367

• NCBI Gene Symbol:

  Dusp1

• Protein Name:

  Q64623

• Gene Name URL:

  Dusp1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_053769.3