DUSP1 ELISA Kit (Rat)

CAT: 0247-OKEH05930-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH05930-96WSize:96 Tests
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Dual specificity phosphatase 1
Gene Aliases
3CH134;3CH134/CL100 PTPase; CL100; dual specificity protein phosphatase 1; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; mitogen-activated protein kinase phosphatase 1; Mkp1; MKP-1; oxidative stress-inducible protein tyrosine phosphatase; protein-tyrosine phosphatase CL100; protein-tyrosine phosphatase non-receptor type 16; Ptpn16.
Gene ID
114856
Accession Number
NP_446221.2
Reactivity
Rat, Rattus norvegicus
Target
Dual specificity protein phosphatase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dusp1 ELISA Kit (Rat) (OKEH05930) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dusp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dusp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dusp1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 102%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Dual specificity protein phosphatase 1
Protein Length
367
NCBI Gene Symbol
Dusp1
Protein Name
Q64623
Gene Name URL
Dusp1
Nucleotide Accession Number
NM_053769.3

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