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LAPTM4B ELISA Kit (Mouse)

CAT: 0247-OKEH05922-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05922-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Lysosomal-associated protein transmembrane 4B
Gene Aliases
C330023P13Rik; LAPTM4beta; lysosomal-associated transmembrane protein 4; lysosomal-associated transmembrane protein 4B.
Gene ID
114128
Accession Number
NP_277056.1
Reactivity
Mouse, Mus musculus
Target
Lysosomal-associated transmembrane protein 4B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Laptm4b ELISA Kit (Mouse) (OKEH05922) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Laptm4b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Laptm4b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Laptm4b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.7%
Sensitivity
41.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Lysosomal-associated transmembrane protein 4B
Protein Length
227
NCBI Gene Symbol
Laptm4b
Protein Name
Q91XQ6
Gene Name URL
Laptm4b
Nucleotide Accession Number
NM_033521.3

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