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SLC12A2 ELISA Kit (Mouse)

CAT: 0247-OKEH05865-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05865-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Solute carrier family 12, member 2
Gene Aliases
9330166H04R;9330166H04Rik; basolateral Na-K-Cl symporter; bumetanide-sensitive sodium- (potassium) -chloride cotransporter 1; bumetanide-sensitive sodium- (potassium) -chloride cotransporter 2; mBSC; mBSC2; Nkcc; Nkcc1; sodium/potassium/chloride cotransporters; sodium/potassium/chloride transporters; solute carrier family 12 member 2; sy; sy-ns.
Gene ID
20496
Accession Number
NP_033220.2
Reactivity
Mouse, Mus musculus
Target
Solute carrier family 12 member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc12a2 ELISA Kit (Mouse) (OKEH05865) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc12a2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc12a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc12a2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 98.9%
Sensitivity
39.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Solute carrier family 12 member 2
Protein Length
1205
NCBI Gene Symbol
Slc12a2
Protein Name
P55012
Gene Name URL
Slc12a2
Nucleotide Accession Number
NM_009194.3

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