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BIRC2 ELISA Kit (Mouse)

CAT: 0247-OKEH05703-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH05703-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Baculoviral IAP repeat-containing 2
Gene Aliases
A; Api1; Api2; apoptosis inhibitor 2; AW146227; baculoviral IAP repeat-containing 3; baculoviral IAP repeat-containing protein 2; Birc3; C330006D17Rik; cellular inhibitor of apoptosis 1; cI; cIA; cIAP1; C-IAP1; cIAP2; HI; HIAP1; HIAP2; I; IAP1; IAP2; IAP-2; inhibitor of apoptosis protein 2; mcI; mcIAP1; MI; MIAP1; mIAP-2; MIAP2; MIH; MIHB; MIHC; RING-type E3 ubiquitin transferase BIRC2; RNF48.
Gene ID
11797
Accession Number
NP_001278432.1
Reactivity
Mouse, Mus musculus
Target
Baculoviral IAP repeat-containing protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Birc2 ELISA Kit (Mouse) (OKEH05703) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Birc2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Birc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Birc2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 96.4%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Baculoviral IAP repeat-containing protein 2
Protein Length
612
NCBI Gene Symbol
Birc2
Protein Name
Q62210
Gene Name URL
Birc2
Nucleotide Accession Number
NM_001291503.1

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