BIRC2 ELISA Kit (Human)

CAT: 0247-OKEH02117-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02117-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Baculoviral IAP repeat containing 2
Gene Aliases
API1; apoptosis inhibitor 1; baculoviral IAP repeat-containing protein 2; cellular inhibitor of apoptosis 1; cIAP1; c-IAP1; Hiap-2; HIAP2; IAP homolog B; IAP-2; inhibitor of apoptosis protein 2; MIHB; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; RNF48; TNFR2-TRAF-signaling complex protein 2.
Gene ID
329
Accession Number
NP_001157.1
Reactivity
Homo sapiens, Human
Target
Baculoviral IAP repeat-containing protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BIRC2 ELISA Kit (Human) (OKEH02117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BIRC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BIRC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BIRC2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
70 kDa
Specificity
Natural and recombinant Human Baculoviral IAP repeat-containing protein 2
Protein Length
618
NCBI Gene Symbol
BIRC2
Protein Name
Q13490
Gene Name URL
BIRC2
Nucleotide Accession Number
NM_001166.4

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