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CA4 ELISA Kit (Mouse)

CAT: 0247-OKEH05683-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05683-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Carbonic anhydrase 4
Gene Aliases
AW456718; Ca4; CA-IV; carbonate dehydratase IV; carbonic anhydrase 4; carbonic anhydrase IV.
Gene ID
12351
Accession Number
NP_031633.1
Reactivity
Mouse, Mus musculus
Target
Carbonic anhydrase 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ca4 ELISA Kit (Mouse) (OKEH05683) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ca4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ca4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ca4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97.4%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Carbonic anhydrase 4
Protein Length
305
NCBI Gene Symbol
Car4
Protein Name
Q64444
Gene Name URL
Car4
Nucleotide Accession Number
NM_007607.2

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