EIF2AK2 ELISA Kit (Mouse)

CAT: 0247-OKEH05663-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05663-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Eukaryotic translation initiation factor 2-alpha kinase 2
Gene Aliases
2310047A08Rik;4732414G15Rik; AI467567; AI747578; dsRNA-activated kinase; eIF-2 alpha; eIF-2A protein kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible RNA-dependent protein kinase; P; P1/eIF-2A protein kinase; p68 kinase; Pkr; Prkr; protein kinase R; protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; serine/threonine-protein kinase TIK; T-cell viral integration site; Ti; Tik; tyrosine-protein kinase EIF2AK2.
Gene ID
19106
Accession Number
NP_035293.1
Reactivity
Mouse, Mus musculus
Target
Interferon-induced, double-stranded RNA-activated protein kinase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Eif2ak2 ELISA Kit (Mouse) (OKEH05663) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eif2ak2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Eif2ak2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eif2ak2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 91.7%
Sensitivity
0.157 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase
Protein Length
515
NCBI Gene Symbol
Eif2ak2
Protein Name
Q03963
Gene Name URL
Eif2ak2
Nucleotide Accession Number
NM_011163.4

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