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NLGN3 ELISA Kit (Mouse)

CAT: 0247-OKEH05617-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05617-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Neuroligin 3
Gene Aliases
A230085M13Rik; gliotactin homolog; HN; HNL3; N; neuroligin 3 protein; neuroligin-3; NL; NL3; NLG3.
Gene ID
245537
Accession Number
NP_766520.2
Reactivity
Mouse, Mus musculus
Target
Neuroligin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nlgn3 ELISA Kit (Mouse) (OKEH05617) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlgn3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlgn3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlgn3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Notes
Formerly GWB-J5R3UP
Specificity
Natural and recombinant Mouse Neuroligin-3
Protein Length
825
NCBI Gene Symbol
Nlgn3
Protein Name
Q8BYM5
Gene Name URL
Nlgn3
Nucleotide Accession Number
NM_172932.4

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