FOXC2 ELISA Kit (Mouse)

CAT: 0247-OKEH05599-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH05599-96WSize:96 Tests
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Forkhead box C2
Gene Aliases
BF-3; brain factor 3; Fkh14; forkhead box protein C2; forkhead homolog 14; forkhead-related protein FKHL14; Hfhbf3; mesenchyme fork head protein 1; Mfh1; MFH-1; transcription factor FKH-14.
Gene ID
14234
Accession Number
NP_038547.2
Reactivity
Mouse, Mus musculus
Target
Forkhead box protein C2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Foxc2 ELISA Kit (Mouse) (OKEH05599) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Foxc2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Foxc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Foxc2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 82.5%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Forkhead box protein C2
Protein Length
494
NCBI Gene Symbol
Foxc2
Protein Name
Q61850
Gene Name URL
Foxc2
Nucleotide Accession Number
NM_013519.2

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