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MOCOS ELISA Kit (Mouse)

CAT: 0247-OKEH05553-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05553-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Molybdenum cofactor sulfurase
Gene Aliases
MCS, MOS, HMCS, 1110018O12Rik
Gene ID
68591
Accession Number
NP_081055.1
Reactivity
Mouse
Target
Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form.UniRule annotation
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Mocos ELISA Kit (Mouse) (OKEH05553) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mocos has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mocos is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mocos captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 91.3%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Molybdenum cofactor sulfurase
Protein Length
862
NCBI Gene Symbol
MOCOS
Protein Name
Molybdenum cofactor sulfurase
Gene Name URL
MOCOS
Nucleotide Accession Number
NM_026779.1

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