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LAMA3 ELISA Kit (Mouse)

CAT: 0247-OKEH05552-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05552-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Laminin, alpha 3
Gene Aliases
[a]3; [a]3B; epiligrin subunit alpha; kalinin subunit alpha; Lama3B; laminin subunit alpha-3; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; nicein subunit alpha; nicein, 150kDa.
Gene ID
16774
Accession Number
NP_034810.1
Reactivity
Mouse, Mus musculus
Target
Laminin subunit alpha-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lama3 ELISA Kit (Mouse) (OKEH05552) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lama3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lama3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lama3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, urine, cell culture supernatants and other biological fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.797 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Laminin subunit alpha-3
Protein Length
3330
NCBI Gene Symbol
Lama3
Protein Name
Q61789
Gene Name URL
Lama3
Nucleotide Accession Number
NM_010680.1

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