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ARHGAP21 ELISA Kit (Mouse)

CAT: 0247-OKEH05408-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05408-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Rho GTPase activating protein 21
Gene Aliases
5530401C11Rik; AA416458; ARHG; ARHGAP10; rho GTPase-activating protein 10; rho GTPase-activating protein 21; rho-type GTPase-activating protein 21.
Gene ID
71435
Accession Number
NP_001074833.3
Reactivity
Mouse, Mus musculus
Target
Rho GTPase-activating protein 21
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Arhgap21 ELISA Kit (Mouse) (OKEH05408) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Arhgap21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Arhgap21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Arhgap21 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 106.3%
Sensitivity
40.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Rho GTPase-activating protein 21
Protein Length
1944
NCBI Gene Symbol
Arhgap21
Protein Name
Q6DFV3
Gene Name URL
Arhgap21
Nucleotide Accession Number
NM_001081364.3

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