BSG ELISA Kit (Mouse)

CAT: 0247-OKEH05316-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05316-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Basigin
Gene Aliases
5A11/Basigin; AI115436; AI325119; basic immunoglobulin superfamily; basigin; CD147; EMM; EMMPRIN; gp 42; HT-7; HT7 antigen; membrane glycoprotein gp42; neuro; neurothelin.
Gene ID
12215
Accession Number
NP_001070652.1
Reactivity
Mouse, Mus musculus
Target
Basigin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Bsg ELISA Kit (Mouse) (OKEH05316) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bsg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bsg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bsg captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99.1%
Sensitivity
41 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Basigin
Protein Length
389
NCBI Gene Symbol
Bsg
Protein Name
P18572
Gene Name URL
Bsg
Nucleotide Accession Number
NM_001077184.1

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