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TFPI2 ELISA Kit (Mouse)

CAT: 0247-OKEH05291-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05291-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Tissue factor pathway inhibitor 2
Gene Aliases
AV000670; PP5/TF; PP5/TFPI-2; tissue factor pathway inhibitor 2.
Gene ID
21789
Accession Number
NP_033390.1
Reactivity
Mouse, Mus musculus
Target
Tissue factor pathway inhibitor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tfpi2 ELISA Kit (Mouse) (OKEH05291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tfpi2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tfpi2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tfpi2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 112%
Sensitivity
15.72 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Tissue factor pathway inhibitor 2
Protein Length
230
NCBI Gene Symbol
Tfpi2
Protein Name
O35536
Gene Name URL
Tfpi2
Nucleotide Accession Number
NM_009364.4

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