HSD3B1 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

• Gene Aliases:

  3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I;3-beta-HSD I;3-beta-hydroxy-5-ene steroid dehydrogenase;3-beta-hydroxy-Delta (5) -steroid dehydrogenase;3-beta-hydroxysteroid 3-dehydrogenase; D3Ertd383; D3Ertd383e; delta-5-3-ketosteroid isomerase; dihydrotestosterone oxidoreductase; steroid Delta-isomerase.

• Gene ID:

  15492

• Accession Number:

  NP_001291729.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Hsd3b1 ELISA Kit (Mouse) (OKEH05151) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsd3b1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsd3b1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsd3b1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  78-5000pg/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 102.2%

• Sensitivity:

  39 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

• Protein Length:

  373

• NCBI Gene Symbol:

  Hsd3b1

• Protein Name:

  P24815

• Gene Name URL:

  Hsd3b1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001304800.1