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HSD3B1 ELISA Kit (Human)

CAT: 0247-OKEH01670-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01670-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Gene Aliases
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I;3BETAHSD;3-beta-HSD I;3-beta-hydroxy-5-ene steroid dehydrogenase;3-beta-hydroxy-Delta (5) -steroid dehydrogenase;3-beta-hydroxysteroid 3-dehydrogenase; delta-5-3-ketosteroid isomerase; dihydrotestosterone oxidoreductase; HSD3B; HSDB3; HSDB3A; progesterone reductase; SDR11E1; short chain dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; trophoblast antigen FDO161G.
Gene ID
3283
Accession Number
NP_000853.1
Reactivity
Homo sapiens, Human
Target
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology HSD3B1 ELISA Kit (Human) (OKEH01670) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human HSD3B1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HSD3B1 and incubated. The HSD3B1 found in the sample or standards competes with the biotinylated HSD3B1 for limited binding sites on the immobilized anti-human HSD3B1 antibody. Excess unbound biotinylated HSD3B1 and sample or standard HSD3B1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HSD3B1 captured in the well and inversely proportional to the amount of HSD3B1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Protein Length
373
NCBI Gene Symbol
HSD3B1
Protein Name
P14060
Gene Name URL
HSD3B1
Nucleotide Accession Number
NM_000862.2

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