PDLIM1 ELISA Kit (Mouse)

CAT: 0247-OKEH05138-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05138-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
PDZ and LIM domain 1 (elfin)
Gene Aliases
Carboxyl terminal LIM domain protein 1; Clim1; CLP; CLP36; C-terminal LIM domain protein 1; elfin; LIM domain protein CLP-36; mCli; mClim1; PDZ and LIM domain protein 1.
Gene ID
54132
Accession Number
NP_058557.2
Reactivity
Mouse, Mus musculus
Target
PDZ and LIM domain protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pdlim1 ELISA Kit (Mouse) (OKEH05138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdlim1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdlim1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdlim1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 87.4%
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse PDZ and LIM domain protein 1
Protein Length
327
NCBI Gene Symbol
Pdlim1
Protein Name
O70400
Gene Name URL
Pdlim1
Nucleotide Accession Number
NM_016861.4

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