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TIRAP ELISA Kit (Mouse)

CAT: 0247-OKEH05102-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05102-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
Gene Aliases
AA407980; adaptor protein Wyatt; C130027E04Rik; M; Mal; myD88 adapter-like protein; TIR domain-containing adapter protein; Tlr4ap; toll/interleukin-1 receptor domain-containing adapter protein; Toll-like receptor 4 adaptor protein; wyat; Wyatt.
Gene ID
117149
Accession Number
NP_473437.2
Reactivity
Mouse, Mus musculus
Target
Toll/interleukin-1 receptor domain-containing adapter protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tirap ELISA Kit (Mouse) (OKEH05102) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tirap has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tirap is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tirap captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99.6%
Sensitivity
15.7 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Toll/interleukin-1 receptor domain-containing adapter protein
Protein Length
241
NCBI Gene Symbol
Tirap
Protein Name
Q99JY1
Gene Name URL
Tirap
Nucleotide Accession Number
NM_054096.2

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