TNFRSF12A ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  Tumor necrosis factor receptor superfamily, member 12a

• Gene Aliases:

  AI255180; C87282; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; fibroblast growth factor-regulated protein 2; Fn1; Fn14; HPIP; tumor necrosis factor receptor superfamily member 12A; Twea; TWEAK; TweakR; TWEAK-R; tweak-receptor; type I transmembrane protein Fn14.

• Gene ID:

  27279

• Accession Number:

  NP_001155218.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Tumor necrosis factor receptor superfamily member 12A

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Tnfrsf12a ELISA Kit (Mouse) (OKEH05034) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf12a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tnfrsf12a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf12a captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 99%

• Sensitivity:

  0.078 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 12A

• Protein Length:

  129

• NCBI Gene Symbol:

  Tnfrsf12a

• Protein Name:

  Q9CR75

• Gene Name URL:

  Tnfrsf12a

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001161746.1