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IL3RA ELISA Kit (Mouse)

CAT: 0247-OKEH04994-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04994-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Interleukin 3 receptor, alpha chain
Gene Aliases
CD123; CDw123; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3RA; IL-3R-alpha; interleukin-3 receptor class II alpha chain; interleukin-3 receptor subunit alpha; SUT-; SUT-1.
Gene ID
16188
Accession Number
NP_032395.1
Reactivity
Mouse, Mus musculus
Target
Interleukin-3 receptor subunit alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Il3ra ELISA Kit (Mouse) (OKEH04994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il3ra has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il3ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il3ra captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 101.5%
Sensitivity
15.72 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Interleukin-3 receptor subunit alpha
Protein Length
396
NCBI Gene Symbol
Il3ra
Protein Name
P26952
Gene Name URL
Il3ra
Nucleotide Accession Number
NM_008369.1

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