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SLC27A5 ELISA Kit (Human)

CAT: 0247-OKEH04989-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04989-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Solute carrier family 27 member 5
Gene Aliases
ACSB; ACSVL6; BA-CoA ligase; BACS; BAL; bile acid-CoA ligase; bile acyl-CoA synthetase; cholate--CoA ligase; FACVL3; FATP5; FATP-5; fatty acid transport protein 5; fatty-acid-Coenzyme A ligase, very long-chain 3; solute carrier family 27 (fatty acid transporter), member 5; Solute carrier family 27 member 5; very long-chain acyl-CoA synthetase homolog 2; very long-chain acyl-CoA synthetase-related protein; VLACSR; VLACS-related; VLCSH2; VLCS-H2.
Gene ID
10998
Accession Number
NP_001308125.1
Reactivity
Homo sapiens, Human
Target
Bile acyl-CoA synthetase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC27A5 ELISA Kit (Human) (OKEH04989) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC27A5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC27A5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC27A5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Bile acyl-CoA synthetase
Protein Length
690
NCBI Gene Symbol
SLC27A5
Protein Name
Q9Y2P5
Gene Name URL
SLC27A5
Nucleotide Accession Number
NM_001321196.1

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