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TSHR ELISA Kit (Mouse)

CAT: 0247-OKEH04953-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04953-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Thyroid stimulating hormone receptor
Gene Aliases
AI481368; hypot; hypothroid; hyt; pe; pet; Thyroid-stimulating hormone receptor; thyrotropin receptor; TSH-R.
Gene ID
22095
Accession Number
NP_035778.3
Reactivity
Mouse, Mus musculus
Target
Thyrotropin receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tshr ELISA Kit (Mouse) (OKEH04953) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tshr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tshr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tshr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 112.6%
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Thyrotropin receptor
Protein Length
764
NCBI Gene Symbol
Tshr
Protein Name
P47750
Gene Name URL
Tshr
Nucleotide Accession Number
NM_011648.5

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