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CPM ELISA Kit (Human)

CAT: 0247-OKEH04872-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04872-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Carboxypeptidase M
Gene Aliases
Carboxypeptidase M; renal carboxypeptidase; urinary carboxypeptidase B.
Gene ID
1368
Accession Number
NP_001005502.1
Reactivity
Homo sapiens, Human
Target
Carboxypeptidase M
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CPM ELISA Kit (Human) (OKEH04872) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPM captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100ng/mL
Sensitivity
0.67 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
51 kDa
Specificity
Natural and recombinant Human Carboxypeptidase M
Protein Length
443
NCBI Gene Symbol
CPM
Protein Name
P14384
Gene Name URL
CPM
Nucleotide Accession Number
NM_001005502.2

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