PDGFRB ELISA Kit (Human)

CAT: 0247-OKEH04766-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04766-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Platelet derived growth factor receptor beta
Gene Aliases
Activated tyrosine kinase PDGFRB; Beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; IBGC4; IMF1; JTK12; KOGS; NDEL1-PDGFRB; PDGFR; PDGFR1; PDGFR-1; PDGFR-beta; PDGF-R-beta; PENTT; platelet-derived growth factor receptor 1; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, beta polypeptide.
Gene ID
5159
Accession Number
NP_002600.1
Reactivity
Homo sapiens, Human
Target
Platelet-derived growth factor receptor beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDGFRB ELISA Kit (Human) (OKEH04766) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDGFRB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDGFRB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDGFRB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
124 kDa
Specificity
Natural and recombinant Human Platelet-derived growth factor receptor beta
Protein Length
1106
NCBI Gene Symbol
PDGFRB
Protein Name
P09619
Gene Name URL
PDGFRB
Nucleotide Accession Number
NM_002609.3

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