Reg4 ELISA Kit (Mouse)

CAT: 0247-OKEH04715-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04715-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Regenerating islet-derived family, member 4
Gene Aliases
2010002L15Rik; GISP; R; REG-4; regenerating islet-derived protein 4; RELP.
Gene ID
67709
Accession Number
NP_080604.2
Reactivity
Mouse, Mus musculus
Target
Regenerating islet-derived protein 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Reg4 ELISA Kit (Mouse) (OKEH04715) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Reg4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Reg4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Reg4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100ng/mL
Sensitivity
0.82 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 kDa
Specificity
Natural and recombinant Mouse Regenerating islet-derived protein 4
Protein Length
157
NCBI Gene Symbol
Reg4
Protein Name
Q9D8G5
Gene Name URL
Reg4
Nucleotide Accession Number
NM_026328.2

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