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Nts ELISA Kit (Mouse)

CAT: 0247-OKEH04658-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04658-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Neurotensin
Gene Aliases
5033428E16Rik; neuromedin N; neurotensin/neuromedin N; NMN-1; NMN-125; NN; NT; NT/N; NTS1.
Gene ID
67405
Accession Number
NP_077755.1
Reactivity
Mouse, Mus musculus
Target
Neurotensin/neuromedin N
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Nts ELISA Kit (Mouse) (OKEH04658) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Nts antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nts and incubated. The found in the sample or standards competes with the biotinylated Nts for limited binding sites on the immobilized anti-Mouse Nts antibody. Excess unbound biotinylated Nts and sample or standard Nts is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nts captured in the well and inversely proportional to the amount of Nts which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
41.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 KDa
Specificity
Natural and recombinant Mouse Neurotensin/neuromedin N
Protein Length
169
NCBI Gene Symbol
Nts
Protein Name
Q9D3P9
Gene Name URL
Nts
Nucleotide Accession Number
NM_024435.2

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