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TOP2B ELISA Kit (Human)

CAT: 0247-OKEH04656-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04656-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
DNA topoisomerase II beta
Gene Aliases
Antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen.
Gene ID
7155
Accession Number
NP_001059.2
Reactivity
Homo sapiens, Human
Target
DNA topoisomerase 2-beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TOP2B ELISA Kit (Human) (OKEH04656) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TOP2B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TOP2B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TOP2B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.045 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
183 KDa
Specificity
Natural and recombinant Human DNA topoisomerase 2-beta
Protein Length
1612
NCBI Gene Symbol
TOP2B
Protein Name
Q02880
Gene Name URL
TOP2B
Nucleotide Accession Number
NM_001068.3

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