Anpep ELISA Kit (Mouse)

• Gene Name:

  Alanyl (membrane) aminopeptidase

• Gene Aliases:

  Alanyl aminopeptidase; aminopeptidase M; aminopeptidase N; aminopeptidase N/CD13; AP-M; Apn; AP-N; Cd13; membrane protein p161; microsomal aminopeptidase; P150.

• Gene ID:

  16790

• Accession Number:

  NP_032512.2

• Reactivity:

  Mouse, Mus musculus

• Target:

  Aminopeptidase N

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Anpep ELISA Kit (Mouse) (OKEH04605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anpep has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Anpep is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anpep captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.78-50ng/mL

• Sensitivity:

  0.41 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  110 KDa

• Specificity:

  Natural and recombinant Mouse Aminopeptidase N

• Protein Length:

  966

• NCBI Gene Symbol:

  Anpep

• Protein Name:

  P97449

• Gene Name URL:

  Anpep

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_008486.2